Wells of microtiter plates coated with antiseptic FGF10 antibody, the solid appearance are again able samples taken at anniversary microplate or accepted biotinylated FGF10 antibody, HRP-labeled avidin, done thoroughly with the substrate (TMB) color. TMB peroxidase catalyses the about-face in the blue, and the role of acerbic in the final yellow.
The abyss and blush samples FGF10 were absolutely correlated. Then, by allegory of the absorbance (OD) is determined, the adding of the sample absorption at 450 nm wavelength.
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